Journal: PLOS One

Article Title: Down-regulation of circ0001361 induces apoptosis and suppresses the progression of glioma

doi: 10.1371/journal.pone.0343681

Figure Lengend Snippet: (A) MiRNAs targeted by circ0001361 were predicted. (B) Expression of hsa-miR-525-5p in glioma (Group A) and normal brain tissue (Group B) was shown. (C) Expression of hsa-miR-525-5p in glioma grade Ⅱ -Ⅳ was shown. (D) Prognostic significance of hsa-miR-525-5p was analyzed. (E) Potential binding sites between circ0001361 and hsa-miR-525-5p were predicted. (F) Circ0001361-hsa-miR-525-5p interaction was confirmed by dual-luciferase reporter assay.

Article Snippet: After 48 hours, the Firefly luciferase and Renilla Luciferase were detected using the Dual Luciferase Reporter Assay Kit (Vazyme, China).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay

Journal: PLOS One

Article Title: Down-regulation of circ0001361 induces apoptosis and suppresses the progression of glioma

doi: 10.1371/journal.pone.0343681

Figure Lengend Snippet: (A) Genes targeted by hsa-miR-525-5p were predicted. (B) Correlation between hsa-miR-525-5p and MEIS1 was analyzed. (C) Prognostic significance of MEIS1 was analyzed. (D) Expression of MEIS1 in GBM, LGG and normal brain tissues was shown. (E) Expression of MEIS1 in glioma grade Ⅱ -Ⅳ was shown. (F) The relationship between MEIS1 and IDH mutation status was shown. (G) The relationship between MEIS1 and 1p/19q co−deletion status was shown. (H) Potential binding sites between hsa-miR-525-5p and MEIS1 were predicted. (I) Hsa-miR-525-5p and MEIS1 interaction was confirmed by dual-luciferase reporter assay.

Article Snippet: After 48 hours, the Firefly luciferase and Renilla Luciferase were detected using the Dual Luciferase Reporter Assay Kit (Vazyme, China).

Techniques: Expressing, Mutagenesis, Binding Assay, Luciferase, Reporter Assay

Journal: Cell reports

Article Title: Nef-Mediated CD3-TCR Downmodulation Dampens Acute Inflammation and Promotes SIV Immune Evasion

doi: 10.1016/j.celrep.2020.01.069

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Luciferase Assay System 10-pack , Promega , Cat# E1501.

Techniques: Virus, Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Cell Isolation, Mutagenesis, DNA Ligation, Control, Luciferase, Cell Culture, Lysis, Gene Expression, Plasmid Preparation, Software

Journal: Disease Markers

Article Title: miR-1 Inhibits Cell Growth, Migration, and Invasion by Targeting VEGFA in Osteosarcoma Cells

doi: 10.1155/2016/7068986

Figure Lengend Snippet: The interaction between miR-1 and VEGFA. (a) Putative binding sites for miR-1 on VEGFA 3′-UTR were shown. The mutated region in VEGFA 3′-UTR was labeled by a horizontal line. (b) U2OS cells were cotransfected with a luciferase reporter containing the wild type (WT) or mutant (Mut) VEGFA 3′-UTR, along with miR-1 mimic or scramble mimic. The relative luciferase activities were measured 24 h after transfection. (c) The protein expression level of VEGFA was detected by western blot. A representative western blot and quantitative results of densitometry are shown. Data were expressed as the mean ± SD ( n = 3). ∗ P < 0.01; NS, no significant difference compared to control.

Article Snippet: The dual luciferase reporter plasmid fused with the wild type or mutant 3′-UTR segment of human VEGFA was purchased from GenePharma, Shanghai, China.

Techniques: Binding Assay, Labeling, Luciferase, Mutagenesis, Transfection, Expressing, Western Blot, Control